Flow Cytometry Core Facility

About the Flow Cytometry Core Facility

The Creighton Flow Cytometry Core Facility is now based in room 383 of the Criss III building on the Creighton campus.  It was started in September of 2001 with the purchase of a Becton Dickinson FACSCalibur.  Dr. Perry was hired in October of that year to run the facility. 

In November of 2005 we acquired a Becton Dickinson FACSAria.  This was the first FACSAria placed worldwide with a UV laser.  This is a state of the art, 3 laser, 15 parameter instrument capable of high-speed sorting.  With this instrument we can do up to 14 colors simultaneously, sort at a rate of up to 30,000 cells/second, and sort 4 different populations from the same sample simultaneously.

In addition, this instrument has the ACDU (Automatic Cell Deposition Unit) cloning attachment, allowing the investigator to deposit single cells (or as many cells as wanted) into individual wells of a microtiter plate.

In the spring of 2016 we updated our instrumentation once again.  We replaced the original 4-color FACSCalibur analyzer with a 15-color Yeti analyzer.  The Yeti is a significant upgrade for our facility as it has both a yellow-green 561nm laser and the ability to perform high throughput screening.  The yellow-green laser is better for many of the PE tandem dyes (such as PE-CF594), but more importantly it opens up an entire new frontier of fluorescent dyes used for molecular biology (the "fruit" dyes, such as mCherry, mPlum, etc.).  For more specific information on the two cytometers in the facility, click on the specific links on the left.

For help in designing panels and antibody combinations click on the links below.