Michael G. Nichols, MS, PhD
Michael G. Nichols, MS, PhD

Michael G. Nichols, MS, PhD

Professor
Program Director,
College of Arts and Sciences

Academic Appointments

Department

  • Physics

Position

  • Professor

Secondary Appointment

  • Radiology

Publications and Presentations

Articles

  • D.E. Desa, M. G. Nichols, H. J. Smith. Aminoglycosides rapidly inhibit NAD(P)H metabolism increasing reactive oxygen species and cochlear cell demise. Accepted - In Press, Journal of Biomedical Optics, September 2018., Journal of Biomedical Optics, 2018
  • I. Meerovich, M. G. Nichols, A.K. Dash. Lipid-based nanoparticles for addressed delivery of paclitaxel with controlled induction of drug release by near-infrared light illumination of low intensity. Journal of Drug Targeting. Submitted September 10, 2018., Journal of Drug Targeting, 2018
  • S.V. Prathivadhi-Bhayankaram, J. Ning, M. Mimlitz, C.Taylor, E. Gross, M. Nichols, J. Guck, and A. E. Ekpenyong. Chemotherapy impedes in vitro microcirculation and promotes migration of leukemic cells with impact on metastasis.  , Biochem. Biophys. Res. Commun., 479, 841-846, 2016
  • M.G. Nichols and R Hallworth. The Single-Molecule Approach to Membrane Protein Stoichiometry. Auditory and Vestibular Research: Methods and Protocols. Volume 1427 of Methods in Molecular Biology. Bernd Sokolowski Ed. pp 189-199, Springer, New York, 2016., Methods in Molecular Biology, 1427, 189-199, 2016
  • Zholudeva, L. V., Ward, K. G., Nichols, M. G., Smith, H. J. Gentamicin differentially alters cellular metabolism of cochlear hair cells as revealed by NAD(P)H fluorescence lifetime imaging, Journal of Biomedical Optics, 20, Article ID 051032, 2015
  • Autofluorescence Lifetime Imaging, CRC Press- Taylor and Francis Group, 2014
  • Ward, Kristina G., Zholudeva, Lana V., Nichols, Michael G., Jensen Smith, Heather Gentamiacin Differentially Affects OHC and IHC Metabolism as Revealed by NADH Fluorescence Lifetime Imaging, Biophysical Journal, 104, 305a, 2013
  • Nichols, Michael G., Zholudeva, Lyandysha V., Lehnerz, Marcus J., Desa, Danielle E., Meyer, Christian T. Metabolic Profiling of Multicell Tumor Spheroids by NADH Fluorescence and Spatially-Resolved Oximetry, Biophysical Journal, 104, 304a, 2013
  • Holman, Nathan, Hauger, Eric, Nichols, Michael, Sidebottom, David, Haas, Eric One and Two Photon Fluorescence Correlation Spectroscopy on Proteins in Glucose Solutions, Biophysical Journal, 104, 346a-347a, 2013
  • Hallworth, Richard, Stark, Kelsey, Zholudeva, Lyandysha V., Currall, Benjamin B., Nichols, Michael G. The Conserved Tetrameric Subunit Stoichiometry of Slc26 Proteins, Microscopy and Microanalysis, 19, 799-807, 2013
  • Hallworth, Richard, Nichols, Michael G. Prestin in HEK cells is an obligate tetramer, Journal of Neurophysiology, 107, 5-11, 2012
  • Vergen, J., Hecht, C., Zholudeva, L. V., Marquardt, M. M., Hallworth, R., Nichols, M. G. Metabolic Imaging Using Two-Photon Excited NADH Intensity and Fluorescence Lifetime Imaging, Microscopy and Microanalysis, 18, 761-770, 2012
  • Subunit Counting as an Approach to Membrane Protein Stoichiometry, Microscopy and Microanalysis, 18, 771-780, 2012
  • Gentamicin rapidly inhibits mitochondrial metabolism in high-frequency cochlear outer hair cells, PLoS One, 7, e38471, 2012
  • Tonniges, J., Hansen, M., Duncan, J., Bassett, M. J., Fritzsch, B., Gray, B. D., Easwaran, A., Nichols, Michael G. Photo- and bio-physical characterization of novel violet and near-infrared lipophilic fluorophores for neuronal tracing, Journal of microscopy, 239, 117-134, 2010
  • Ekpenyong, Andrew E., Posey, Carolyn L., Chaput, Joy L., Burkart, Anya K., Marquardt, Meg M., Smith, Timothy J., Nichols, Michael G. Determination of cell elasticity through hybrid ray optics and continuum mechanics modeling of cell deformation in the optical stretcher, Applied Optics, 48, 6344-6354, 2009
  • Matei, V. A., Feng, F., Pauley, S., Beisel, K. W., Nichols, M. G., Fritzsch, B. Near-infrared laser illumination transforms the fluorescence absorbing X-Gal reaction product BCI into a transparent, yet brightly fluorescent substance, Brain Research Bulletin, 70, 33-43, 2006
  • Tiede, L. M., Nichols, M. G. Photobleaching of reduced nicotinamide adenine dinucleotide and the development of highly fluorescent lesions in rat basophilic leukemia cells during multiphoton microscopy, Photochemistry and Photobiology, 82, 656-664, 2006
  • Barth, E. E., Hallworth, R., Nichols, M. G. A comparison of the sensitivity of photodamage assays in rat basophilic leukemia cells., Photochem Photobiol, 81, 556-562, 2005

Presentations

  • M.G. Nichols. , K. Brittan, D.L. Che, M. F. Gabriel, M. Glick, B. Huerter, K. Jackson, P. Khoury, D. Myers, P. Nguyen, O. Oladokun, D. L. Pham, K. Sotelo, B. Yang, L. Hansen. In vivo imaging of NAD(P)H by phasor FLIM to monitor changes in metabolism associated with UV‐induced skin cancer. NE-INBRE 16th Annual Conference, August 6, 2018, Nebraska City, NE, 2018
  • M.G. Nichols. The Integrated Biomedical Imaging Core Facility. NE-INBRE 16th Annual Conference, August 6, 2018, Nebraska City, NE, 2018
  • M. Waid, L. Aumen, J. List, N. Cohorst, M. G. Nichols, A. Csordas, J. Schubert. Operator Exposure in Interventional Radiology. American Association of Physicists in Medicine (AAPM) Annual Meeting, July 29, 2018 Nashville, TN, 2018
  • K.D. Sotelo, K.A. Jackson, M.F. Gabriel, C.R. Miller, A.M. Mills, O.C. Oladokun, D.L. Pham, B.L. Yang, L.A. Hansen, and M.G. Nichols. Two-photon imaging of NAD(P)H in squamous cell carcinoma to assess metabolic changes due to HER2 using phasor FLIM. National IDEA symposium of Biomedical Research Excellence (NISBRE), June 24– 26, 2018, Washington, D.C., 2018
  • M.G. Nichols, K Brittan, M.F. Gabriel, M. Glick, K. Jackson, P. Khoury, P. Nguyen, O. Oladokun, D.L. Pham, K.D. Sotelo, B. Yang, Development of an multiphoton laser-scanning imaging platform for in situ metabolic imaging of disease progression in skin cancer by NAD(P)H FLIM, NE-INBRE 15th Annual Conference, August 6, 2017, Nebraska City, NE, 2017
  • D.L. Pham, K.D. Sotelo, M.F. Gabriel and Michael G. Nichols, Applying Phasor FLIM to assess the metabolic impact of ultraviolet exposure and HER2 inhibition in cultured Squamous Cell Carcinoma and in vivo skin. 2017 Central Region IDeA Meeting, June 8, 2017. Sioux Falls, SD, 2017
  • D. R. Cerutis, M.G. Nichols, S.A. Khan, T. Miyamoto, Localization of the Platelet-Activating Factor Receptor on the Intact Human Periodontal Ligament, American Association of Anatomists Annual Meeting at Experimental Biology 2017,  April 26, 2017, Chicago, IL, 2017
  • M.G. Nichols, R. Das, M.F. Gabriel, C.R. Miller, A.M. Mills, D.M. Myers, D.L. Pham, I.N. Pociupany, K. D. Sotelo, K. E. Troup Metabolic imaging of Squamous cell carcinoma cells and in vivo skin by phasor FLIM. Biophysical Society 61st Annual Meeting, February 13, 2017, New Orleans, LA, 2017
  • M.G. Nichols, M.F. Gabriel, C.R. Miller, A.M. Mills, D.M. Myers,  D.L. Pham, I.N. Pociupany, K.D.  Sotelo, K.E. Troup, Progress toward metabolic imaging of disease progression in skin cancer by FLIM, NE-INBRE 14th Annual Conference, August 7, 2016, Nebraska City, NE, 2016
  • D. Desa, E. Riley, M.G. Nichols, H. Jensen Smith. Ototoxic Aminoglycosides inhibit NADH metabolism and increase reactive oxygen species formation in Cochlear cells. Biophysical Society 60th Annual Meeting, March 2, 2016, Los Angeles, CA., 2016
  • E. Effa, A. Ikpeme, M. Inyama, B. Agu, T. Ekpenyong, A. Otu, N. Ani,W. Egbe, E.A. Tom, S. Prathivadhi, M.G. Nichols, S. Kosoko-Lasaki, A. Ekpenyong. Mobile Clinics Deployed in Rural Nigeria for Free Patient Care and Clinical Research. Global Health Conference Midwest, February 5, 2016, Omaha, NE., 2016
  • C. Taylor , S. Prathivadhi, J. Ning, M.G. Nichols, A. Ekpenyong. Microfluidic devices for biomechanical assessment of metastatic effects of cancer drugs. Global Health Conference Midwest, February 5, 2016, Omaha, NE., 2016
  • The Role of Complex I in Mitochondrial Reactive Oxygen Species Formation in Cochlear Sensory and Supporting Cells During Aminoglycoside Exposure, 2015
  • The Role of Complex I in Mitochondrial Reactive Oxygen Species Formation in Cochlear Sensory and Supporting Cells During Aminoglycoside Exposure, 2015
  • Metabolic profiling of the skin to monitor the onset and progression of squamous cell carcinoma through time- and wavelength-resolved fluorescence lifetime imaging, 2015
  • Shining a light on cellular metabolism of skin cancer through time- and wavelength-resolved multiphoton microscopy, 2014
  • Label free imaging of the regenerating human periodontal ligament and gingival tissue using second harmonic generation confocal microscopy, 2014
  • Evaluating mitochondrial superoxide formation in cochlear cells during ototoxic antibiotic exposure, 2014
  • Metabolic profiling of cochlear dysfunction via two-photon fluorescence lifetime microscopy of NADH, 2014
  • Mitochondrial respiration studies suggest a novel mechanism for Aminoglycoside-induced hearing loss, 2014
  • Label free imaging of the regenerating human periodontal ligament and gingival tissue using second harmonic generation confocal microscopy, 2014
  • Metabolic Profiling of Cultured Murine Cochleae via Fluorescence Lifetime Microscopy, 2014
  • Differences in Cochlear Sensory and Supporting Cell Mitochondrial Metabolism Bias Free Radical Production, 2014
  • Endogenous Differences in Cochlear Sensory and Supporting Cell Mitochondrial Metabolism Bias Free Radical Production during Ototoxin Exposure, 2014
  • Two-Photon Fluorescence Lifetime Imaging of Cochlear Dysfunction, 2014
  • Metabolic Profiling of Cultured Murine Cochleae via Fluorescence Lifetime Microscopy, 2013
  • Assessing the Impact of Antibiotics on Mitochondrial Respiration, 2013
  • Measuring Cellular Refractive Indicies with Digital Holographic Microscopy in the Near Infrared, 2013
  • Measuring Cellular Refractive Indicies with Digital Holographic Microscopy in the Near Infrared, 2013
  • Metabolic Profiling of Cochlear Dysfunction by Fluorescence Lifetime Microscopy, 2013
  • Metabolic Profiling of Cochlear Dysfunction by Fluorescence Lifetime Microscopy, 2013
  • The Single-Molecule Approach to Membrane Protein Stoichiometry, 2013
  • Advanced Methods in Microscopy, 2013
  • Metabolic Profiling of Cochlear Dysfunction by Fluorescence Lifetime Microscopy, 2013
  • Assessing cellular metabolism by NADH FLIM and intensity-based measurements of monolayers and multicell tumor spheroids, 2013
  • Design and construction of a spatially-resolved oximeter for metabolic profiling of multicell tumor spheroids, 2013
  • Ototoxic antibiotic-induced changes in cochlear metabolism generate cell-damaging free radicals, 2013
  • Spatially resolved profiling of multicell spheroids by NADH fluorescence lifetime imaging microscopy, 2013
  • Two-photon fluorescence correlation spectroscopy of proteins in glucose solutions, 2013
  • Design and construction of a spatially-resolved oximeter for metabolic profiling of multicell tumor spheroids, 2013
  • Biophysical imaging with multiphoton microscopy, 2013
  • The Conserved Tetrameric Subunit Stoichiometry of Slc26 Proteins, 2013
  • Gentamicin Differentially Affects OHC and IHC Metabolism as Revealed by NADH Fluorescence Lifetime Imaging, 2013
  • Metabolic profiling of multicell tumor spheroids by NADH fluorescence and spatially-resolved oximetry, 2013
  • One and Two Photon Fluorescence Correlation Spectroscopy on Proteins in Glucose Solutions, 2013
  • Metabolic Imaging using Two-Photon excited NADH Fluorescence Intensity and FLIM, 2012
  • Fluorescence correlation spectroscopy of proteins in sugar solutions, 2012
  • Intensity- and lifetime-based analyses of endogenous differences in NADH metabolism in cochlear hair cells, 2012
  • Two-photon fluorescence intensity and lifetime imaging of NADH reveals glucose availability in cultured cells, 2012
  • Fluorescence Correlation Spectroscopy of Tryptophan-containing Proteins in Sugar Solutions Using Two Photon Excitation, 2012
  • Prestin in Membranes Is a Tetramer, 2012
  • Two-photon fluorescence intensity and lifetime imaging microscopy of cultured cells, 2011

Research and Scholarship

Grant Funding Received

  • Metabolic Imaging of Disease progression in Skin Cancer by FLIM
  • NADH Fluorescence Lifetime-Based Analysis of Mitochondrial Disfunction during Ototoxic Antibiotic Exposure
  • Comparison of NADH FLIM and Intensity Imaging to Assess Cellular Energetics
  • Comparison of NADH FLIM and Intensity Imaging to Assess Cellular Energetics
  • Comparison of NADH FLIM and Intensity Imaging to Assess Cellular Energetics
  • Light Scattering and Fluorescence Study of Sugar Solutions as Cryoprotective Agents in Protein Storage