Barbara J. O'Kane, BS, MS, PhD
Barbara J. O'Kane, BS, MS, PhD

Barbara J. O'Kane, BS, MS, PhD

Associate Professor
School of Dentistry

Academic Appointments

Department

  • Oral Biology

Position

  • Associate Professor

Biography

Dr. Barbara J. O’Kane is an Associate Professor in the Dept. of Oral Biology at Creighton University School of Dentistry in Omaha NE. She is currently Course Director for the D1 Neuroscience and also teaches in the D1 Gross Anatomy and Head and Neck courses. Research areas of interest are mesenchymal stem cells, hematopoietic stem cells and tumor stem cells. She is also involved in educational research for the improvement of learning outcomes for professional students. Prior to joining the faculty at Creighton University, Dr. O’Kane was at the University of Nebraska Medical Center in Omaha NE where she taught in the Gross Anatomy and Histology courses for first year medical students and conducted research in several areas of adult stem cells. Dr. O’Kane received her Ph.D. from the University of Nebraska Medical Center. Her Dissertation was entitled Bone Marrow Metastases in Breast Cancer.

Education

  • University of Nebraska-Lincoln, Lincoln, NE. 1976-1978, B.S. 1978, Natural Resources-Wildlife Biology
  • University of Nebraska Medical Center, Omaha, NE. 1990, M.S.
  • University of Nebraska Medical Center, Omaha, NE. 2000, Ph.D.

Certifications

ASCP Certification – Immunology – 1989 ASCP Certification – Specialty in Immunology – 1993

Publications and Presentations

Articles

  • Farrell, T. L., McGuire, T. R., Bilek, L., Brusnahan, S. K., Jackson, J. D., Lane, J. T., Garvin, K. L., O'Kane, B. J., Berger, A. M., Tuljapurkar, S. R., Kessinger, M. A., Sharp, J. G. Changes in the frequencies of human hematopoietic stem and progenitor cells with age and site, Experimental hematology, 42, 146-154, 2014
  • Barbara J O’Kane, Marcel D DeVetten, John J Jackson, Jordan P Lacy, Tracy L Farrell, John G Sharp. Multipotential mesenchymal stromal cells (MMSC) ameliorate graft versus host disease in a mouse model, but major of GVHD permits leukemic relapse., Stem Cell Discovery, 4, 37-43, 2014
  • Tracy L. Farrell, Timothy R. McGuire, Laura D. Bilek, Susan K. Brusnahan, John D. Jackson, Judy T. Lane, Kevin L. Garvin, Barbara J. O’Kane, Ann M. Berger, Sonal R. Tuljapurkara,M. Anne Kessinger, and John Graham Sharp. Changes in the frequencies of human hematopoietic stem and progenitor cells with age and site., Experimental hematology, 42, 146-154, 2014
  • Mukhopadhyay, Partha, Farrell, Tracy, Sharma, Gayatri, McGuire, Timothy R., O'Kane, Barbara, Sharp, J. G. Heterogeneity of Functional Properties of Clone 66 Murine Breast Cancer Cells Expressing Various Stem Cell Phenotypes, PLoS One, 8, e78725, 2013
  • Mukhopadhyay, Partha, Tracy Farrell, Gayatri Sharma, Timothy McGuire, Barbara O’Kane, J Graham Sharp. Heterogeneity of functional properties of Clone 66 murine breast cancer cells expressing various stem cell phenotypes., PLoS One, Vol 8 (11), 1-15, 2013
  • S Tuljaputkar, J. Jackson, S Brusnahan, B O’Kane & J Sharp. Characterization of a mesenchymal stem cell line that differentiates to bone and provides niches supporting mouse and human hematopoietic cells., Stem Cell Discovery, Vol 2 No 1, 5-14, 2012
  • McGuire TR, Brusnahan SK, Bilek LD, Jackson JD, Kessinger MA, Berger AM, Garvin KL, O’Kane BJ, Tuljapurkar SR, Sharp JG. Inflammation Associated with Obesity: Relationship with Blood and Bone Marrow Endothelial Cells. Obesity 2011, Maternal Child Nursing, 2011
  • Sonal R Tuljapurkar; Susan K Brusnahan; Kevin L Garvin; John D Jackson; Margaret A Kessinger; James T Lane; Timothy R McGuire; Barbara J O' Kane; John G Sharp. Changes in human bone marrow fat content associated with changes in hematopoietic stem cell numbers and cytokine levels with aging., Journal of Anatomy, 219 (5), 574-81, 2011
  • Brushnahan SK., McGuire TR, Jackson JD, Lane JT, Garvin KL, O’Kane BJ, Berger AM, Tuljapukar SR, Kessinger MA, Sharp JG. Human blood and marrow side population stem cell numbers decline with age, with an increase in quality of surviving stem cells: Correlation with cytokines., Mechanisms of Ageing and Development, 131, 718-22, 2010

Presentations

  • PUETZ III, William J., Laura C. Barritt, Jason M. Kum, Sonia M. Sanchez, Shikha Tarang, Barbara J. O’Kane. Department of Oral Biology, School of Dentistry, Creighton University, Omaha, NE 68178, USA. TREM-1 and TREM-2 protein expression in human gingival tissue. University Research Day/St Albert’s Day April 2015, Creighton University, Omaha NE 68178, 2015
  • PUETZ III, William J., Laura C. Barritt, Jason M. Kum, Sonia M. Sanchez, Shikha Tarang, Barbara J. O’Kane. Department of Oral Biology, School of Dentistry, Creighton University, Omaha, NE 68178, USA. TREM-1 and TREM-2 protein expression in human gingival tissue. AACA Henderson, NV. 2015, 2015
  • Kum, Jason M., Laura C. Barritt, William J. Puetz, Sonia M. Sanchez, Shikha Tarang, and Barbara J. O’Kane. Determination of Expression of TREM-1 and TREM-2 in Oral Keratinocytes. Department of Oral Biology, School of Dentistry, Creighton University, Omaha, NE 68178, USA. AACA Henderson NV. 2015., 2015
  • Massie H. Jesse and Barbara J O’Kane. Dept. of Oral Biology, Creighton University School of Dentistry. Primary culture of oral mucosa using the direct explant technique. American Association of Clinical Anatomists. Orlando FL. July 2014, 2014
  • Master of Oral Biology, Anatomic science track: A path to addressing the need for dental educators. Margaret A. Jergenson, Laura C. Barritt, Neil S. Norton, Barbara J. O’Kane and Mark A. Latta. American Association of Anatomists. Orlando FL July 2014., 2014
  • Massie H. Jesse and Barbara J O’Kane. Dept. of Oral Biology, Creighton University School of Dentistry. Primary culture of oral mucosa using the direct explant technique. University Research Day/St Albert’s Day April 2014, Creighton University, Omaha NE 68178, 2014
  • Leukemic Relapse Following MMSC Rescue In A Murine Model Of GVHD Barbara O’Kane, Marcel DeVetten, John Jackson, Jordan Lacy, J Graham Sharp Creighton University School of Dentistry, Omaha NE. University of Nebraska Medical Center, Omaha NE. Wake Forest School of Medicine, Institute of Regenerative Medicine, Winston Salem NC. American Association of Anatomists 2013, Boston Massachusetts, 2013
  • Effect of Various Modes of Exercise on the Mobilization of Stem Cells Barbara O’Kane , Patrick Passarelli, Molly Lang, Susan Brusnahan, Laura Bilek. Dept of Oral Biology Creighton University, Omaha NE and UNMC, Omaha NE. American Association of Anatomists 2011, Washington DC, 2011

Research and Scholarship

Research and Scholarship Interests

  • Research areas of interest are mesenchymal stem cells, hematopoietic stem cells and tumor stem cells.

Current Research Projects

  • Oral stem cells and immunology of the oral cavity Educational research for improving learning outcomes for for professional students

Grant Funding Received

  • Characterization of oral tissue mesenchymal progenitor cells Creighton University Presidents Faculty Research Fund $10,000 2/1/2012 – 1/31/2013 Role: PI
  • Effects of Vitamin-D on the Expression of TREM-1 and TREM-2 by Oral Keratinocytes State of Nebraska LB692 $50,000 2012-2014 Role: PI
  • Longitudinal assessment of basic science retention in dental students. Creighton University Office for Academic Excellence and Assessment $895.00 Role: Co-I Grants funded
  • Effects of Vitamin-D on the Expression of TREM-1 and TREM-2 by Oral Keratinocytes State of Nebraska LB692 $50,000 2012-2014 Role: PI
  • Longitudinal assessment of basic science retention in dental students. Creighton University Office for Academic Excellence and Assessment $895.00 Role: Co-I