Lynne Margaret Dieckman, MS, PhD
Associate Professor
Contact
College of Arts and Sciences
Chemistry
Chemistry Faculty
Biochemistry
RGSB - Rigge Science Building
Lynne Margaret Dieckman, MS, PhD
Associate Professor
Research Focus
Research in the Dieckman lab focuses on the mechanisms by which errors in DNA replication and repair and chromatin organization lead to genome instability and disease. Specifically, our main goal is to characterize the interactions of key factors that are essential for the interplay between genomic and epigenetic stability. Two of these factors include the proteins PCNA (the processivity factor that stimulates DNA polymerases during DNA replication and repair) and CAF-1 (the main histone chaperone that is necessary for packaging DNA into nucleosomes following replication and repair). The interaction between PCNA and CAF-1 is crucial for proper DNA synthesis, assembly of nucleosomes, and preservation of epigenetic marks. Students will learn biochemical techniques used in modern biomedical research in order to determine the mechanistic basis of the network of interactions between CAF-1, PCNA, DNA, and other factors during replication-dependent nucleosome assembly and to work towards an understanding of proper DNA maintenance.
Department
Chemistry and Biochemistry
Position
Associate Professor
Articles
- Progress in Biophysics and Molecular Biology
Lynne Dieckman, Something’s gotta give: How PCNA alters its structure in response to mutations and the implications on cellular processes 2020 - Journal of Biological Chemistry
Salmonella enterica serovar Typhimurium has three transketolase enzymes contributing to the pentose phosphate pathway
293 (29), p. 11271-11282 2018 - PLoS One
Crystal structures of PCNA mutant proteins defective in gene silencing suggest a novel interaction site on the front face of the PCNA ring
13(3), p. e0193333 2018 - McGraw Hill
Aspirin
055900 2017 - PLoS One
Identification of New Mutations at the PCNA Subunit Interface that Block Translesion Synthesis
11(6), p. e0157023 2016 - Nucleic Acids Research
Eukaryotic Y-Family Polymerases: A Biochemical and Structural Perspective
30, p. 85-108 2014 - Biochemistry
Distinct Structural Alterations in PCNA Block DNA Mismatch Repair
52 (33), p. 5611-5619 2013 - DNA Repair
PCNA Trimer Instability Inhibits Translesion Synthesis by DNA Polymerase Eta and by DNA Polymerase Delta
12 (5), p. 367-376 2013 - Springer Science+Business Media LLC
PCNA Structure and Function: Insights from Structures of PCNA Complexes and Post-translationally Modified PCNA
62, p. 281-299 2012 - Biochemistry
Pre-steady State Kinetic Studies of the Fidelity of Nucleotide Incorporation by Yeast DNA Polymerase Delta
49 (34), p. 7344-7350 2010
Other
Dr. George F. Haddix President’s Faculty Research Fund, Creighton University
National Institutes of Health INBRE Program, DRPP
National Science Foundation Nebraska EPSCoR FIRST Award