Research Services

Molecular Biology Research Core Facility

This research core facility provides both services and instrumentation for all investigators at Creighton University who utilize molecular biology techniques in their research. The services provided include DNA sequencing and oligonucleotide synthesis. In addition, faculty, technicians and graduate students have access to some of the most precise technology and equipment available for their individual use on a sign up basis. This equipment includes:

  • Applied Biosystems 7500 Fast Real-Time PCR
  • Konica Medical Film Processor SRX-101
  • Beckman Coulter Ultracentrifuge XL-90
  • Promega Freezer Program
  • Supply Center for Life Technology
  • Thermocyclers AB 9700
  • Centrifuge 5415R

These Molecular Facilities are located in room 382, Criss III.

Available Services and Charges

GENEWIZ Sanger Sequencing

You can setup an account with GeneWiz to get Sanger sequencing.  Learn more here.

Pre-mixed Sequencing Services for Plasmid DNA or Purified PCR Products (you mix the primer and template in the same tube)

  • $6 per reaction for 1-47 reactions submitted in tubes
  • $5 per reaction for 48-93 reactions submitted in 96 well plates*
  • $4 per reaction for 94 or more reactions submitted in 96 well plates*

* Please note that to receive the volume discount listed, samples need to be submitted in 96 well plate format all at the same time.

PCR Purification is an additional $3 per reaction                                                                            

Pre-defined Sequencing Services is an additional +$1 per reaction (Primer and template are provided in separate tubes)

Custom Sequencing Service is an additional +$4 per reaction. (Recommended for sequencing colonies, glycerol stocks, phage plaques)

Same Day DNA Sequencing Service is no additional charge for Creighton!

 

Oligonucleotide Synthesis

For Oligonucleotide pricing please see the order form below.

Required form: OligoOrders.xls 

Email completed forms to the Molecular Group

For More Information

Please direct collaboration or research inquiries to:

Joseph A. Knezetic, Ph.D.
(402) 280-2511
joek@creighton.edu

or

Toni Howard
(402) 280-1895
molecular@creighton.edu