Close MenuClose
Close Menu

Michael G . Nichols, MS, PhD

Program Director, M.S. Physics and M.S. Medical Physics Programs

Professor

Michael Nichols

Contact

College of Arts and Sciences
Graduate School
Medical Sciences (Master of Science)
Physics (Master of Science)
Physics
HLSB - Hixson Lied Science Building

Michael G . Nichols, MS, PhD

Program Director, M.S. Physics and M.S. Medical Physics Programs

Professor

Department

Physics

Position

Professor

Articles

  • Journal of Biomedical Optics
    D.E. Desa, M. G. Nichols, H. J. Smith. Aminoglycosides rapidly inhibit NAD(P)H metabolism increasing reactive oxygen species and cochlear cell demise. Accepted - In Press, Journal of Biomedical Optics, September 2018. 2018
  • Journal of Drug Targeting
    I. Meerovich, M. G. Nichols, A.K. Dash. Lipid-based nanoparticles for addressed delivery of paclitaxel with controlled induction of drug release by near-infrared light illumination of low intensity. Journal of Drug Targeting. Submitted September 10, 2018. 2018
  • Biochem. Biophys. Res. Commun.
    S.V. Prathivadhi-Bhayankaram, J. Ning, M. Mimlitz, C.Taylor, E. Gross, M. Nichols, J. Guck, and A. E. Ekpenyong. Chemotherapy impedes in vitro microcirculation and promotes migration of leukemic cells with impact on metastasis.  
    479, p. 841-846 2016
  • Methods in Molecular Biology
    M.G. Nichols and R Hallworth. The Single-Molecule Approach to Membrane Protein Stoichiometry. Auditory and Vestibular Research: Methods and Protocols. Volume 1427 of Methods in Molecular Biology. Bernd Sokolowski Ed. pp 189-199, Springer, New York, 2016.
    1427, p. 189-199 2016
  • Journal of Biomedical Optics
    Zholudeva, L. V., Ward, K. G., Nichols, M. G., Smith, H. J. Gentamicin differentially alters cellular metabolism of cochlear hair cells as revealed by NAD(P)H fluorescence lifetime imaging
    20, p. Article ID 051032 2015
  • CRC Press- Taylor and Francis Group
    Autofluorescence Lifetime Imaging 2014
  • Biophysical Journal
    Ward, Kristina G., Zholudeva, Lana V., Nichols, Michael G., Jensen Smith, Heather Gentamiacin Differentially Affects OHC and IHC Metabolism as Revealed by NADH Fluorescence Lifetime Imaging
    104, p. 305a 2013
  • Biophysical Journal
    Nichols, Michael G., Zholudeva, Lyandysha V., Lehnerz, Marcus J., Desa, Danielle E., Meyer, Christian T. Metabolic Profiling of Multicell Tumor Spheroids by NADH Fluorescence and Spatially-Resolved Oximetry
    104, p. 304a 2013
  • Biophysical Journal
    Holman, Nathan, Hauger, Eric, Nichols, Michael, Sidebottom, David, Haas, Eric One and Two Photon Fluorescence Correlation Spectroscopy on Proteins in Glucose Solutions
    104, p. 346a-347a 2013
  • Microscopy and Microanalysis
    Hallworth, Richard, Stark, Kelsey, Zholudeva, Lyandysha V., Currall, Benjamin B., Nichols, Michael G. The Conserved Tetrameric Subunit Stoichiometry of Slc26 Proteins
    19, p. 799-807 2013
  • Journal of Neurophysiology
    Hallworth, Richard, Nichols, Michael G. Prestin in HEK cells is an obligate tetramer
    107, p. 5-11 2012
  • Microscopy and Microanalysis
    Vergen, J., Hecht, C., Zholudeva, L. V., Marquardt, M. M., Hallworth, R., Nichols, M. G. Metabolic Imaging Using Two-Photon Excited NADH Intensity and Fluorescence Lifetime Imaging
    18, p. 761-770 2012
  • Microscopy and Microanalysis
    Subunit Counting as an Approach to Membrane Protein Stoichiometry
    18, p. 771-780 2012
  • PLoS One
    Gentamicin rapidly inhibits mitochondrial metabolism in high-frequency cochlear outer hair cells
    7, p. e38471 2012
  • Journal of microscopy
    Tonniges, J., Hansen, M., Duncan, J., Bassett, M. J., Fritzsch, B., Gray, B. D., Easwaran, A., Nichols, Michael G. Photo- and bio-physical characterization of novel violet and near-infrared lipophilic fluorophores for neuronal tracing
    239, p. 117-134 2010
  • Applied Optics
    Ekpenyong, Andrew E., Posey, Carolyn L., Chaput, Joy L., Burkart, Anya K., Marquardt, Meg M., Smith, Timothy J., Nichols, Michael G. Determination of cell elasticity through hybrid ray optics and continuum mechanics modeling of cell deformation in the optical stretcher
    48, p. 6344-6354 2009
  • Brain Research Bulletin
    Matei, V. A., Feng, F., Pauley, S., Beisel, K. W., Nichols, M. G., Fritzsch, B. Near-infrared laser illumination transforms the fluorescence absorbing X-Gal reaction product BCI into a transparent, yet brightly fluorescent substance
    70, p. 33-43 2006
  • Photochemistry and Photobiology
    Tiede, L. M., Nichols, M. G. Photobleaching of reduced nicotinamide adenine dinucleotide and the development of highly fluorescent lesions in rat basophilic leukemia cells during multiphoton microscopy
    82, p. 656-664 2006
  • Photochem Photobiol
    Barth, E. E., Hallworth, R., Nichols, M. G. A comparison of the sensitivity of photodamage assays in rat basophilic leukemia cells.
    81, p. 556-562 2005

Presentations

  • M.G. Nichols. , K. Brittan, D.L. Che, M. F. Gabriel, M. Glick, B. Huerter, K. Jackson, P. Khoury, D. Myers, P. Nguyen, O. Oladokun, D. L. Pham, K. Sotelo, B. Yang, L. Hansen. In vivo imaging of NAD(P)H by phasor FLIM to monitor changes in metabolism associated with UV‐induced skin cancer. NE-INBRE 16th Annual Conference, August 6, 2018, Nebraska City, NE 2018
  • M.G. Nichols. The Integrated Biomedical Imaging Core Facility. NE-INBRE 16th Annual Conference, August 6, 2018, Nebraska City, NE 2018
  • M. Waid, L. Aumen, J. List, N. Cohorst, M. G. Nichols, A. Csordas, J. Schubert. Operator Exposure in Interventional Radiology. American Association of Physicists in Medicine (AAPM) Annual Meeting, July 29, 2018 Nashville, TN 2018
  • K.D. Sotelo, K.A. Jackson, M.F. Gabriel, C.R. Miller, A.M. Mills, O.C. Oladokun, D.L. Pham, B.L. Yang, L.A. Hansen, and M.G. Nichols. Two-photon imaging of NAD(P)H in squamous cell carcinoma to assess metabolic changes due to HER2 using phasor FLIM. National IDEA symposium of Biomedical Research Excellence (NISBRE), June 24– 26, 2018, Washington, D.C. 2018
  • M.G. Nichols, K Brittan, M.F. Gabriel, M. Glick, K. Jackson, P. Khoury, P. Nguyen, O. Oladokun, D.L. Pham, K.D. Sotelo, B. Yang, Development of an multiphoton laser-scanning imaging platform for in situ metabolic imaging of disease progression in skin cancer by NAD(P)H FLIM, NE-INBRE 15th Annual Conference, August 6, 2017, Nebraska City, NE 2017
  • D.L. Pham, K.D. Sotelo, M.F. Gabriel and Michael G. Nichols, Applying Phasor FLIM to assess the metabolic impact of ultraviolet exposure and HER2 inhibition in cultured Squamous Cell Carcinoma and in vivo skin. 2017 Central Region IDeA Meeting, June 8, 2017. Sioux Falls, SD 2017
  • D. R. Cerutis, M.G. Nichols, S.A. Khan, T. Miyamoto, Localization of the Platelet-Activating Factor Receptor on the Intact Human Periodontal Ligament, American Association of Anatomists Annual Meeting at Experimental Biology 2017,  April 26, 2017, Chicago, IL 2017
  • M.G. Nichols, R. Das, M.F. Gabriel, C.R. Miller, A.M. Mills, D.M. Myers, D.L. Pham, I.N. Pociupany, K. D. Sotelo, K. E. Troup Metabolic imaging of Squamous cell carcinoma cells and in vivo skin by phasor FLIM. Biophysical Society 61st Annual Meeting, February 13, 2017, New Orleans, LA 2017
  • M.G. Nichols, M.F. Gabriel, C.R. Miller, A.M. Mills, D.M. Myers,  D.L. Pham, I.N. Pociupany, K.D.  Sotelo, K.E. Troup, Progress toward metabolic imaging of disease progression in skin cancer by FLIM, NE-INBRE 14th Annual Conference, August 7, 2016, Nebraska City, NE 2016
  • D. Desa, E. Riley, M.G. Nichols, H. Jensen Smith. Ototoxic Aminoglycosides inhibit NADH metabolism and increase reactive oxygen species formation in Cochlear cells. Biophysical Society 60th Annual Meeting, March 2, 2016, Los Angeles, CA. 2016
  • E. Effa, A. Ikpeme, M. Inyama, B. Agu, T. Ekpenyong, A. Otu, N. Ani,W. Egbe, E.A. Tom, S. Prathivadhi, M.G. Nichols, S. Kosoko-Lasaki, A. Ekpenyong. Mobile Clinics Deployed in Rural Nigeria for Free Patient Care and Clinical Research. Global Health Conference Midwest, February 5, 2016, Omaha, NE. 2016
  • C. Taylor , S. Prathivadhi, J. Ning, M.G. Nichols, A. Ekpenyong. Microfluidic devices for biomechanical assessment of metastatic effects of cancer drugs. Global Health Conference Midwest, February 5, 2016, Omaha, NE. 2016
  • The Role of Complex I in Mitochondrial Reactive Oxygen Species Formation in Cochlear Sensory and Supporting Cells During Aminoglycoside Exposure 2015
  • The Role of Complex I in Mitochondrial Reactive Oxygen Species Formation in Cochlear Sensory and Supporting Cells During Aminoglycoside Exposure 2015
  • Metabolic profiling of the skin to monitor the onset and progression of squamous cell carcinoma through time- and wavelength-resolved fluorescence lifetime imaging 2015
  • Shining a light on cellular metabolism of skin cancer through time- and wavelength-resolved multiphoton microscopy 2014
  • Label free imaging of the regenerating human periodontal ligament and gingival tissue using second harmonic generation confocal microscopy 2014
  • Evaluating mitochondrial superoxide formation in cochlear cells during ototoxic antibiotic exposure 2014
  • Metabolic profiling of cochlear dysfunction via two-photon fluorescence lifetime microscopy of NADH 2014
  • Mitochondrial respiration studies suggest a novel mechanism for Aminoglycoside-induced hearing loss 2014
  • Label free imaging of the regenerating human periodontal ligament and gingival tissue using second harmonic generation confocal microscopy 2014
  • Metabolic Profiling of Cultured Murine Cochleae via Fluorescence Lifetime Microscopy 2014
  • Differences in Cochlear Sensory and Supporting Cell Mitochondrial Metabolism Bias Free Radical Production 2014
  • Endogenous Differences in Cochlear Sensory and Supporting Cell Mitochondrial Metabolism Bias Free Radical Production during Ototoxin Exposure 2014
  • Two-Photon Fluorescence Lifetime Imaging of Cochlear Dysfunction 2014
  • Metabolic Profiling of Cultured Murine Cochleae via Fluorescence Lifetime Microscopy 2013
  • Assessing the Impact of Antibiotics on Mitochondrial Respiration 2013
  • Measuring Cellular Refractive Indicies with Digital Holographic Microscopy in the Near Infrared 2013
  • Measuring Cellular Refractive Indicies with Digital Holographic Microscopy in the Near Infrared 2013
  • Metabolic Profiling of Cochlear Dysfunction by Fluorescence Lifetime Microscopy 2013
  • Metabolic Profiling of Cochlear Dysfunction by Fluorescence Lifetime Microscopy 2013
  • The Single-Molecule Approach to Membrane Protein Stoichiometry 2013
  • Advanced Methods in Microscopy 2013
  • Metabolic Profiling of Cochlear Dysfunction by Fluorescence Lifetime Microscopy 2013
  • Assessing cellular metabolism by NADH FLIM and intensity-based measurements of monolayers and multicell tumor spheroids 2013
  • Design and construction of a spatially-resolved oximeter for metabolic profiling of multicell tumor spheroids 2013
  • Ototoxic antibiotic-induced changes in cochlear metabolism generate cell-damaging free radicals 2013
  • Spatially resolved profiling of multicell spheroids by NADH fluorescence lifetime imaging microscopy 2013
  • Two-photon fluorescence correlation spectroscopy of proteins in glucose solutions 2013
  • Design and construction of a spatially-resolved oximeter for metabolic profiling of multicell tumor spheroids 2013
  • Biophysical imaging with multiphoton microscopy 2013
  • The Conserved Tetrameric Subunit Stoichiometry of Slc26 Proteins 2013
  • Gentamicin Differentially Affects OHC and IHC Metabolism as Revealed by NADH Fluorescence Lifetime Imaging 2013
  • Metabolic profiling of multicell tumor spheroids by NADH fluorescence and spatially-resolved oximetry 2013
  • One and Two Photon Fluorescence Correlation Spectroscopy on Proteins in Glucose Solutions 2013
  • Metabolic Imaging using Two-Photon excited NADH Fluorescence Intensity and FLIM 2012
  • Fluorescence correlation spectroscopy of proteins in sugar solutions 2012
  • Intensity- and lifetime-based analyses of endogenous differences in NADH metabolism in cochlear hair cells 2012
  • Two-photon fluorescence intensity and lifetime imaging of NADH reveals glucose availability in cultured cells 2012
  • Fluorescence Correlation Spectroscopy of Tryptophan-containing Proteins in Sugar Solutions Using Two Photon Excitation 2012
  • Prestin in Membranes Is a Tetramer 2012
  • Two-photon fluorescence intensity and lifetime imaging microscopy of cultured cells 2011

State

  • NADH Fluorescence Lifetime-Based Analysis of Mitochondrial Disfunction during Ototoxic Antibiotic Exposure

Federal

  • Comparison of NADH FLIM and Intensity Imaging to Assess Cellular Energetics

  • Comparison of NADH FLIM and Intensity Imaging to Assess Cellular Energetics

  • Comparison of NADH FLIM and Intensity Imaging to Assess Cellular Energetics

  • Light Scattering and Fluorescence Study of Sugar Solutions as Cryoprotective Agents in Protein Storage