Flow Cytometry Core

The Flow Cytometry Core Facility is based in the Department of Medical Microbiology and Immunology, and is located in room 383 of the Dr. C.C. and Mabel L. Criss Health Sciences Complex III.  The core provides equipment and services to identify, quantify, analyze, and isolate cells in complex mixtures (like blood or tissue).  The facility is equipped with two state-of-the-art flow cytometers:

  1. BioRAD ZE5 (Yeti) Cell Analyzer: up to 15-color analysis and High Throughput Screening.
  2. Invitrogen Bigfoot Spectral Cell Sorter: for full-spectrum  analysis and high-speed cell sorting.
     

The facility also has a workstation setup as a data server and data analysis station for our users.  All computers in the facility are connected to the Creighton network and have both black & white and color laser printers.  All data generated in the facility is routinely backed up and saved.

Feel free to reach out to the core at flow@creighton.edu or stop by for a free consultation. Find out how you can utilize flow cytometry in your lab.

Acknowledgements

The facility gratefully acknowledges funding from the NIH (3R01GM102487-03S1) and the LB692 Nebraska Tobacco Settlement Biomedical Research Development Program to P.C.S. to support the acquisition of the BioRAD ZE5 Cell Analyzer, and funding from the NIH (3P20GM139762-04S1) and the Creighton University School of Medicine to P.S.S and P.C.S to support the acquisition of the Invitrogen Bigfoot Spectral Cell Sorter.

Key Personnel

Patrick Swanson

Patrick C. Swanson, PhD