Patrick C . Swanson, PhD

Program Director, Graduate Program Director Department of Medical Microbiology and Immunology

Professor

P Swanson

Contact

School of Medicine
Med Microbiology & Immunology
Graduate School
Medical Microbiology and Immunology (Doctorate)
Medical Microbiology and Immunology (Master of Science)
Pharmaceutical Sciences (Master of Science)
Translational Hearing
Cytometry
CRISS II - Criss 2 - 432

Patrick C . Swanson, PhD

Program Director, Graduate Program Director Department of Medical Microbiology and Immunology

Professor

Cancer Research Focus

The products of the Recombination Activating Genes (RAG) initiate DNA double-strand breaks during V(D)J recombination, which is the process that is responsible for assembling immunoglobulin and T cell receptor genes in lymphocytes.  While essential for normal development of adaptive immunity, this process is not always benign, as certain types of leukemia and lymphoma harbor aberrant DNA rearrangements that can be traced to mistakes during V(D)J recombination.  A major focus of research in my laboratory to understand the regulatory mechanisms that constrain RAG activity and guide the appropriate repair of the RAG-induced DNA breaks to prevent genome instability and cancer.

Cancer Research Area(s)
  • Blood Cancers
Examples of the most recent publications can be obtained from this PubMed link.

Research Focus

I am generally interested in mechanisms of immune repertoire diversification.  My research program is currently focused on studying various aspects of V(D)J recombination, the process by which immunoglobulin and T cell receptor genes are assembled during lymphocyte development.  V(D)J recombination is initiated by the RAG1 and RAG2 proteins, which together introduce DNA breaks in the antigen receptor loci, which are subsequently repaired by components of the non-homologous DNA end-joining (NHEJ) repair pathway (Fig. 1).  The laboratory has two major ongoing projects.  The first project is broadly focused on how proteins that associated with the full-length RAG1 regulate V(D)J recombination by facilitating DNA end-joining or the timely degradation of RAG1.  The second project involves determining whether enforced expression of catalytically inactive RAG1 in mice can impair secondary V(D)J recombination events triggered by engagement of self-antigen (receptor editing) or in response to immunization (receptor revision), and whether defects in these processes increase susceptibility to malignancy or autoimmune disease.  These research projects are or have been supported by both intramural and extramural grants, including grants awarded by the National Institutes of Health and the American Cancer Society.

Department

Med. Microbiology & Immunology

Position

Professor

Books

  • Reference Module in Biomedical Research
    Swanson Patrick C., V(D)J Recombination, Somatic Hypermutation, and Class Switch Recombination [Book Chapter] 2014
  • V(D)J Recombination
    Swanson Patrick C., Early steps of V(D)J rearrangement [Book Chapter] 2009

Articles

  • Swanson Patrick C., Erratum 2003

Publications

  • Nature communications
    Deng Silu, Cryptosporidium uses CSpV1 to activate host type I interferon and attenuate antiparasitic defenses
    14:1, p. 1456 - 17 2023
  • Cell Reports
    Worth Alexandra N., Receptor editing constrains development of phosphatidyl choline-specific B cells in VH12-transgenic mice
    39:11 2022
  • PloS one
    Schabla N. Max, The CRL4VPRBP(DCAF1) E3 ubiquitin ligase directs constitutive RAG1 degradation in a non-lymphoid cell line
    16:10 October 2021
  • Journal of molecular cell biology
    Schabla N. Max, DCAF1 (VprBP)
    11:9, p. 725 - 735 2019
  • Cellular Immunology
    Palmer Victoria L., IL10 restrains autoreactive B cells in transgenic mice expressing inactive RAG1
    331, p. 110 - 120 2018
  • Journal of Immunology
    Max Schabla N., VprBP (DCAF1) regulates RAG1 expression independently of dicer by mediating RAG1 degradation
    201:3, p. 930 - 939 2018
  • Gao Q., Driver Fusions and Their Implications in the Development and Treatment of Human Cancers
    23:1, p. 227 - 238.e3 2018
  • FASEB journal : official publication of the Federation of American Societies for Experimental Biology
    Ma Shibin, A long non-coding RNA, lincRNA-Tnfaip3, acts as a co-regulator of NF-B to modulate inflammatory gene transcription
    31:3, p. 1215 - 1225 2017
  • Cell Reports
    Haney Staci L., Promoter Hypomethylation and Expression Is Conserved in Mouse Chronic Lymphocytic Leukemia Induced by Decreased or Inactivated Dnmt3a
    15:6, p. 1190 - 1201 2016
  • Experimental Hematology
    Shukla A., Absence of Caveolin-1 Leads to Delayed Development of Chronic Lymphocytic Leukemia in Emu-TCL1 Mouse Model
    44:1, p. 30 - 37.e1 2016
  • BMC Immunology
    Palmer Victoria L., Cd1d regulates B cell development but not B cell accumulation and IL10 production in mice with pathologic CD5(+) B cell expansion
    16:66, p. (12 pages) 2015
  • Journal of Immunology
    Palmer Victoria L., VprBP is required for efficient editing and selection of Igκ+ B cells, but is dispensable for Igλ+ and marginal zone B cell maturation and selection
    195:4, p. 1524 - 1537 2015
  • Antioxidants and Redox Signaling
    Manning Jared, Vitamin C promotes maturation of T-cells
    19:17, p. 2054 - 2067 2013
  • BMC Molecular Biology
    Nakagawa Tadashi, VprBP (DCAF1)
    14 2013
  • Blood
    Nganga Vincent K., Accelerated progression of chronic lymphocytic leukemia in Eµ-TCL1 mice expressing catalytically inactive RAG1
    121:19, p. 3855 - 3866 2013
  • Nucleic Acids Research
    Wang Guannan, Real-time monitoring of RAG-catalyzed DNA cleavage unveils dynamic changes in coding end association with the coding end complex
    40:13, p. 6082 - 6096 2012
  • Molecular and Cellular Biology
    Shimazaki Noriko, Mechanistic basis for RAG discrimination between recombination
    32:2, p. 365 - 375 2012
  • Journal of Proteome Research
    Ma Danjun, Smoke-Induced Signal Molecules in Bone Marrow Cells from Altered Low-Density Lipoprotein Receptor-Related Protein 5 Mice
    11:7, p. 3548 - 3560 2012
  • The Journal of immunology (1950)
    Bai Jirong, Molecular regulation of BCL2 to IGH translocation in follicular lymphoma (165.38)
    186:1_Supplement, p. 165 - 165.38 2011
  • The Journal of immunology (1950)
    Xie Wanqin, The V(D)J recombination machinery is organized as large complexes anchored on the nuclear matrix (62.1)
    186:1_Supplement, p. 62 - 62.1 2011
  • PLoS One
    Palmer V. L., N-Acetylcysteine Increases the Frequency of Bone Marrow Pro-B/Pre-B Cells, but Does Not Reverse Cigarette Smoking-Induced Loss of This Subset
    6:9, p. e24804 2011
  • Immunology
    Hassaballa A. E., Accumulation of B1-like B cells in transgenic mice over-expressing catalytically inactive RAG1 in the periphery
    134:4, p. 469 - 486 2011
  • EMBO Journal
    Kassmeier Michele D., VprBP binds full-length RAG1 and is required for B-cell development and V(D)J recombination fidelity
    31:4, p. 945 - 958 2011
  • Annual Review of Genetics
    Schatz David G., V(D)J recombination
    45, p. 167 - 202 2011
  • Inhalation Toxicology
    Fusby J. S., Cigarette smoke-induced effects on bone marrow B-cell subsets and CD4(+):CD8(+) T-cell ratios are reversed by smoking cessation: Influence of bone mass on immune cell response to and recovery from smoke exposure
    22:9, p. 785 - 796 2010
  • The Journal of immunology (1950)
    Lange Miles, The V(D)J recombination machinery is associated with the nuclear matrix (88.3)
    184:1_Supplement, p. 88 - 88.3 2010
  • The Journal of immunology (1950)
    Swanson Patrick, Smoking cessation reverses cigarette smoke-induced impairment of B cell development and skewed CD4:CD8 T cell ratios in mice (36.43)
    184:1_Supplement, p. 36 - 36.43 2010
  • Journal of Biological Chemistry
    Shlyakhtenko Luda S., Molecular mechanism underlying RAG1/RAG2 synaptic complex formation
    284:31, p. 20956 - 20965 2009
  • Nucleic Acids Research
    Kumar Sushil, Full-length RAG1 promotes contact with coding and intersignal sequences in RAG protein complexes bound to recombination signals paired in cis
    37:7, p. 2211 - 2226 2009
  • BMC Molecular Biology
    Zhang Ming, HMGB1/2 can target DNA for illegitimate cleavage by the RAG1/2 complex
    10:1, p. 24 2009
  • Molecular Cell
    Lu Haihui, A Biochemically Defined System for Coding Joint Formation in V(D)J Recombination
    31:4, p. 485 - 497 2008
  • BMC Molecular Biology
    Kriatchko Aleksei N., HMG-box domain stimulation of RAG1
    9, p. 32 - 32 2008
  • Journal of Biological Chemistry
    Swanson Patrick C., V(D)J Recombinase Binding and Cleavage of Cryptic Recombination Signal Sequences Identified from Lymphoid Malignancies
    283:11, p. 6717 - 6727 2008
  • Nucleic Acids Research
    Raval Prafulla, Evidence for Ku70/Ku80 association with full-length RAG1, p. 2060 - 2072 2008
  • Molecular and Cellular Biology
    Ciubotaru Mihai, Fluorescence resonance energy transfer analysis of recombination signal sequence configuration in the RAG1/2 synaptic complex
    27:13, p. 4745 - 4758 2007
  • DNA Repair
    Raghavan Sathees C., The structure-specific nicking of small heteroduplexes by the RAG complex
    6:6, p. 751 - 759 2007
  • The Journal of immunology (1950)
    Zhang Ming, Recognition and cleavage of cryptic recombination signal sequences identified from lymphoid malignancies (49.20)
    178:1_Supplement, p. S86 - S87 2007
  • The Journal of immunology (1950)
    Swanson Patrick C., Immunodeficiency and accumulation of B1-like B cells in transgenic mice overexpressing catalytically inactive RAG1 (B2)
    178:1_Supplement, p. LB1 - LB1 2007
  • Molecular and Cellular Biology
    Kriatchko Aleksei N., Identification and characterization of a gain-of-function RAG-1 mutant
    26:12, p. 4712 - 4728 2006
  • Journal of allergy and clinical immunology
    Parks K.W., Transgenic Mice Expressing Catalytically Inactive RAG-1 Exhibit Hypogammaglobulinemia and Altered B Cell Development, but not Autoreactivity
    117:2, p. S100 - S100 2006
  • Methods in Enzymology
    Bergeron Serge, RAG and HMGB1 Proteins
    408, p. 511 - 528 2006
  • Journal of Biological Chemistry
    Bergeron Serge, Both high mobility group (HMG)-boxes and the acidic tail of HMGB1 regulate recombination-activating gene (RAG)-mediated recombination signal synapsis and cleavage in vitro
    280:35, p. 31314 - 31324 2005
  • Molecular and Cellular Biology
    Raghavan Sathees C., Double-strand break formation by the RAG complex at the Bcl-2 major breakpoint region and at other non-B DNA structures in vitro
    25:14, p. 5904 - 5919 2005
  • Immunological Reviews
    Swanson Patrick C., The bounty of RAGs: recombination signal complexes and reaction outcomes
    200, p. 90 - 114 2004
  • Nature
    Raghavan Sathees C., A non-B-DNA structure at the Bcl-2 major breakpoint region is cleaved by the RAG complex
    428:6978, p. 88 - 93 2004
  • Journal of Biological Chemistry
    Swanson Patrick C., Full-length RAG-2, and Not Full-length RAG-1, Specifically Suppresses RAG-mediated Transposition but Not Hybrid Joint Formation or Disintegration
    279:6, p. 4034 - 4044 2004
  • Molecular and Cellular Biology
    Swanson Patrick C., A RAG-1/RAG-2 tetramer supports 12/23-regulated synapsis, cleavage, and transposition of V(D)J recombination signals (vol 22, pg 7790, 2002) Erratum
    23:8, p. 3029 - 3029 2003
  • Molecular and Cellular Biology
    Swanson Patrick C., A RAG-1/RAG/RAG-2 tetramer supports 12/23-regulated synapsis, cleavage, and transposition of V(D)J recombination signals
    22:22, p. 7790 - 7801 2002
  • Molecular and Cellular Biology
    Swanson Patrick C., Fine structure and activity of discrete RAG-HMG complexes on V(D)J recombination signals
    22:5, p. 1340 - 1351 2002
  • Molecular and Cellular Biology
    Swanson P. C., The DDE motif in RAG-1 is contributed in trans to a single active site that catalyzes the nicking and transesterification steps of V(D)J recombination
    21:2, p. 449 - 458 2001
  • Molecular and Cellular Biology
    Swanson Patrick C., RAG-2 promotes heptamer occupancy by RAG-1 in the assembly of a V(D)J initiation complex
    19:5, p. 3674 - 3683 1999
  • Immunity
    Swanson Patrick C., V(D)J recombination signal recognition
    9:1, p. 115 - 125 1998
  • Molecular and Cellular Biology
    Li Wenhui, RAG-1- and RAG-2-dependent assembly of functional complexes with V(D)J recombination substrates in solution
    17:12, p. 6932 - 6939 1997
  • Journal of the American Chemical Society
    Stevens Shawn Y., Non nucleic acid inhibitors of protein·DNA interactions identified through combinatorial chemistry
    118:43, p. 10650 - 10651 1996
  • Journal of Clinical Investigation
    Swanson Patrick C., Ligand recognition by murine anti-DNA autoantibodies
    97:7, p. 1748 - 1760 1996
  • Biochemistry
    Swanson Patrick C., Ligand recognition by anti-DNA autoantibodies. Affinity, specificity, and mode of binding
    35:5, p. 1624 - 1633 1996
  • Journal of Immunological Methods
    Stevens Shawn Y., Application of the gel shift assay to study the affinity and specificity of anti-DNA autoantibodies
    177:1-2, p. 185 - 190 1994
  • Tetrahedron Letters
    Goodwin Jay T., Synthesis of a disulfide cross-linked DNA triple helix
    35:26, p. 4527 - 4530 1994
  • Journal of Immunology
    Swanson Patrick C., High resolution epitope mapping of an anti-DNA autoantibody using model DNA ligands
    152:5, p. 2601 - 2612 1994
  • Bioorganic and Medicinal Chemistry Letters
    Swanson Patrick C., Chemical sequencing of disulfide-crosslinked oligodeoxyribonucleotides
    3:10, p. 2117 - 2118 1993
  • Journal of the American Chemical Society
    Stevens Shawn Y., Evidence for Induced Fit in Antibody-DNA Complexes
    115:4, p. 1585 - 1586 1993

Presentations

  • Invited Speaker, VA Friday Seminar Series, VprBP(DCAF1) regulates V(D)J recombination by mediating RAG1 degradation: implications to immune repertoire development and genome stability 2019
  • Invited speaker, GCBA Seminar Series, UNMC. VprBP(DCAF1) regulates V(D)J recombination by mediating RAG1 degradation 2018
  • Poster presentation: IL10 regulates in vivo antibody production and in vitro lipopolysaccharide-induced B cell responses in dnRAG1 mice Swanson PC, Palmer VL, Worth A, Scott R, and Perry GA Keystone Symposia "B cells: mechanisms of immunity and autoimmunity" Dresden Germany 2018
  • Poster presentation: VprBP/DCAF1 regulates RAG1 expression independently of Dicer by promoting RAG1 degradation. Schabla NM, Palmer VL, Perry GA, and Swanson PC. Keystone Symposia "B cells: Mechanisms in Immunity and Autoimmunity" Dresden, Germany 2018
  • Oral Presentation: VprBP/DCAF1 regulates V(D)J recombination by mediating RAG1 degradation 2018
  • FASEB SRC "Molecular Mechanisms of Immune Cell Development and Function" Snowmass Village CO Short talk Title: VprBP/DCAF1 mediates post-transcriptional regulation of RAG1 independent of Dicer 2017
  • Poster presentation. "IL10 regulates in vivo antibody production and in vitro lipopolysaccharide-induced B cell responses in dnRAG1 miceFASEB SRC "Molecular mechansism of immune cell development and function" Snowmass Village CO 2017
  • Poster presentation: VprBP regulates RAG1 expression at the post-transcriptional level and independently of Dicer Schabla NM, Palmer VL, Perry GA, and Swanson PC FASEB SRC "Molecular mechansism of immune cell development and function" Snowmass Village CO 2017
  • St. Albert's Day Poster Session, Creighton University. Poster Presentation. N. MAX SCHABLA, Greg A. Perry, James T. Fletcher, Patrick C. Swanson. "STRUCTURAL AND FUNCTIONAL CHARACTERIZATION OF VPRBP, A NOVEL RAG1-INTERACTING FACTOR" 2015
  • Allergy and Immunology Grand Rounds. Creighton University Omaha NE. Oral Presentation. Immunoglobulins: from genetics to B cell biology. 2015
  • 56th American Society for Hematology Annual Meeting and Exposition. San Francisco CA. Poster Presentation. Ashima Shukla, Christine E Cutucache, Karan Rai, Siddharth Rai, Rene Opavsky, Patrick Swanson, and Shantaram S Joshi. Absence of Caveloin-1 Leads to Delayed Development of Chronic Lymphocytic Leukemia in E-TCL1 Mouse Model 2014
  • Great Plains Analytical Cytometry Association 7th Annual Conference. Creighton University Omaha NE. Oral Presentation. "Using Multicolor Flow Cytometry And Cell Sorting To Define A Role For VprBP In B Cell Development And V(D)J Recombination". 2014
  • Cold Spring Harbor Laboratories 2014 Meeting on GENE EXPRESSION & SIGNALING IN THE IMMUNE SYSTEM. Poster Presentation. Victoria L. Palmer, Razia Aziz-Seible, Mary Rothermund, Greg A. Perry, Patrick C. Swanson. VprBP, an E3 ubiquitin ligase adaptor protein that associates with full-length RAG1, is required for efficient V(D)J rearrangement of distal VH and Vk gene segments and editing of Igκ chain, but not for development of Igλ+ B cells 2014
  • GCBA Seminar Series, UNMC, Omaha NE. Oral Presentation. Monoclonal B cell lymphocytosis and chronic lymphocytic leukemia: possible origin due to defects in B cell receptor editing? 2014
  • Midwest Student Biomedical Research Forum, Omaha NE. Poster Presentation. Razia S. Aziz-Seible, Koushik Mondal, Vicky L. Palmer, Greg A. Perry and Patrick C. Swanson. Is Herc2 required for B cell development and V(D)J recombination? 2013
  • School of Pharmacy Seminar Series, UNMC, Omaha NE. Oral Presentation "A RAG1-associating E3 ubiquitin ligase regulates B cell development and V(D)J recombination" 2012
  • CUSOM Faculty Club Reception, Omaha NE. S Kumar and PC Swanson. Poster Presentation. "Evidence for long-range RAG1/2 interactions with coding sequences in discrete V(D)J initiation complexes assembled on coupled cleavage substrates" 2008
  • 39th Annual Midwest Student Biomedical Research Forum, Omaha NE. AN Kriatchko, S Bergeron, and PC Swanson. Oral Presentation. "HMG-box domain stimulation of RAG1/2 cleavage activity is metal ion dependent" 2008
  • 39th Annual Midwest Student Biomedical Research Forum, Omaha NE. M Zhang, S Bergeron and PC Swanson. Poster Presentation. "HMGB1 promotes structure-specific DNA cleavage by the RAG proteins via a nick-hairpin mechanism" 2008
  • 39th Annual Midwest Student Biomedical Research Forum, Omaha NE. S Kumar and PC Swanson. Poster Presentation. "Identification of long range protein-DNA contacts in synaptic complexes involved in V(D)J recombination" 2008
  • Keystone Synposium "DNA Replication and Recombination" Santa Fe, NM Oral Presentation "Evidence for long-range RAG1/2 interactions with coding sequences in discrete V(D)J initiation complexes assembled on coupled cleavage substrates" 2008
  • Keystone Synposium "DNA Replication and Recombination" Santa Fe, NM. Poster Presentation. PC Swanson, M Zhang, and S Bergeron. "HMGB1 promotes structure-specific DNA cleavage by the RAG proteins via a nick-hairpin mechanism." 2008
  • Keystone Synposium "DNA Replication and Recombination" Santa Fe, NM. Poster Presentation. S Kumar and PC Swanson "Evidence for long-range RAG1/2 interactions with coding sequences in discrete V(D)J initiation complexes assembled on coupled cleavage substrates" 2008
  • Molecular and Cellular Biology REsearch Group Oral Presentation "Linking DNA breaks to DNA repair in V(D)J recombination" 2007
  • CUSOM Innovation in Basic Science and Clinical Research, Joselyn Art Museum Omaha NE. P Raval, AN Kriatchko, and PC Swanson. Poster Presentation. "Evidence for Ku70/80 association with full-length RAG1" 2007
  • CUSOM Innovation in Basic Science and Clinical Research, Joselyn Art Museum Omaha NE. SK Anderson, AE Hassaballa, K Parks, GA Perry and PC Swanson. Poster Presentation. "Immunodeficiency and accumulation of B1-like B cells in transgenic mice overexpressing catalytically inactive RAG1" 2007
  • UNMC Department of Pathology/Microbiology and Center for Leukemia and Lymphoma Research. "Links between V(D)J recombination and lymphoid cell malignancy: mechanisms and models" 2007
  • 94th Annual Meeting of the American Association of Immunologists. Miami, FL. PC Swanson, DK Anderson, AE Hassaballa, K Parks, and GA Perry. Poster Presentation. "Immunodeficiency and accumulation of B1-like B cells in transgenic mice overexpressing catalytically inactive RAG1" 2007
  • 94th Annual Meeting of the American Association of Immunologists. Miami, FL. P Raval, S Kumar, AN Kriatchko, and PC Swanson. Poster Presentation. "Evidence for Ku70/Ku80 association with full-length RAG1" 2007
  • 94th Annual Meeting of the American Association of Immunologists. Miami, FL. M Zhang and PC Swanson. Poster Presentation. "Recognition and cleavage of cryptic recombination signal sequences identified from lymphoid malignancies" 2007
  • St. Albert's Day Poster Session, Creighton University. M Zhang and PC Swanson. Poster Presentation. "Recognition and cleavage of cryptic recombination signal sequences identified from lymphoid malignancies" 2007
  • St. Albert's Day Poster Session, Creighton University. AN Kriatchko, DK Anderson, and PC Swanson. Poster Presentation. "Identification of a gain-of-function RAG1 mutant" 2007
  • 3rd Annual Nebraska Research and Innovation Conference. Poster Presentation. PC Swanson and GA Perry. "The Creighton University Flow Cytometry Core Facility: Machines and methods for high speed cell analysis and cell sorting." Quest Center Omaha, NE 2007
  • Oral Presentation "Molecular Science at Creighton University: A brief overview" Nebraska Biomedical Research Retreat, Quarry Oaks, NE 2006
  • EPSCoR 2006 Expo, Lincoln, NE Business Display for Creighton University Flow Cytometry Core Facility Perry, GA and PC Swanson 2006
  • ASM Conference "Mobile DNA" Banff, Alberta, Canada "Characterization of a Novel Gain-of-Function RAG-1 Mutant" 2006
  • 37th Midwest Student Biomedical Research Forum, Omaha NE AE Hassaballa (graduate student) Oral Presentation "Characterization of transgenic mice expressing catalytically inactive recombination activating gene 1" 2006
  • 37th Midwest Student Biomedical Research Forum, Omaha NE P Raval (postdoctoral fellow), S Kumar (postdoctoral fellow), and PC Swanson. Poster Presentation "Association of full-length RAG1 to Ku links the cleavage and joining phases of V(D)J recombination" 2006
  • 37th Midwest Student Biomedical Research Forum, Omaha NE A Kriatchko (graduate student), DK Anderson, and PC Swanson Poster Presentation "Characterization of a novel gain-of-function RAG-1 mutant" 2006
  • 37th Midwest Student Biomedical Research Forum, Omaha NE M Zhang (graduate student) and PC Swanson Poster Presentation "Recognition and cleavage of cryptic recombination signal sequences identified from lymphoid malignancies" 2006
  • Creighton University Department of Medicine Research Conference Omaha, NE "Autoimmunity and Immunodeficiency: A New Model Provides Insights into the Role of RAG-1" 2005
  • Department of Medical Microbiology and Immunology Special Seminar Perry GA, and PC Swanson "Multicolor Cell Analysis and Cell Sorting Arrives at Creighton University" 2005
  • UNMC Department of Genetics, Cell Biology and Anatomy seminar series. Omaha, NE "B cell development in transgenic mice overexpressing catalytically inactive RAG-1: implications for receptor editing and B1 versus B2 cell fate determination" 2005
  • Oral Presentation Immunology Interest Group, University of Nebraska Medical Center "B cell development in transgenic mice expressing catalytically inactive RAG-1" 2005
  • Bergeron S, Madathipirambil T, and Swanson PC. "Dissecting the structure-function relationship of HMGB1 in the initiation of V(D)J recombination in vitro". Poster presentation. Keystone Symposia: B cell development, function, and disease. Steamboat Springs, CO 2005
  • Hassaballa AE, Perry GA, Parks KW, Anderson D, and Swanson PC. "Accumulation of transitional B cells in transgenic mice expressing catalytically inactive RAG-1". Poster presentation. Keystone Symposia: B cell development, function, and disease. Steamboat Springs, CO 2005
  • Kriatchko AN, and Swanson PC. "Identification of a gain-of-function RAG-1 mutant". Poster presentation. Keystone Symposia: B cell development, function, and disease. Steamboat Springs, CO 2005
  • Oral Presentation Allergy Fellows Teaching Conference "Antibodies: structure, function, origins,and investigations therein" 2005
  • Introductory remarks American Cancer Society Relay for Life, Midtown 2005
  • Oral Presentation, Third Annual Microbiology Meeting, University of Nebraska, Lincoln "Recent Research on the V(D)J Recombinase" 2004
  • INBRE program seminar, Creighton University, Omaha, Nebraska "Antibodies: structure, function and origins of diversity" 2004
  • Hassaballa AE, Wang L, and Swanson PC. "Investigation of the dominant negative effect of catalytically deficient mutant RAG-1 in vivo. Poster presentation. Great Plains States Society for Molecular Biology and Genetics. Quest Center, Omaha, NE 2004
  • Kriatchko, A and Swanson, PC. "Identification of a gain-of-function RAG-1 mutant" Poster presentation. Greaat Plains States Society for Molecular Biology and Genetics. Quest Center, Omaha, NE. 2004
  • Bergeron, S and Swanson, PC. "Dissecting the stucture-function relationship of HMGB1 in the initiation of V(D)J recombination." Poster presentation. Great Plains States Society for Molecular Biology and Genetics. Quest Center, Omaha, NE. 2004
  • Raval, P and Swanson, PC. "P125 copurifies with DNA-PK and shares an immunoreactive domain with Artemis." Poster presentation. Great Plains States Society for Molecular Biology and Genetics. Quest Center, Omaha, NE. 2004
  • BRIN program seminar, Creighton University, Omaha, Nebraska "Antibodies: structure, function and origins of diversity" 2003
  • Poster presentation, AAI Immunology 2003, Denver, Colorado "The 'dispensable' regions of RAG-1 and RAG-2 play separable roles in regulating V(D)J recombinase activity in vitro" 2003
  • Oral presentation, AAI Immunology 2003, Denver, Colorado "The 'dispensable' regions of RAG-1 and RAG-2 play separable roles in regulating V(D)J recombinase activity in vitro" 2003
  • Oral Presentation, Nebraska Wesleyan University, Lincoln, Nebraska "V(D)J recombination and the origins of immune repertoire diversity" 2003
  • Oral Presentation, Nucleic Acids Interest Group, Mahoney State Park "A RAG1/RAG2 tetramer supports synapsis, cleavage, and transposition of V(D)J recombination signals" 2002
  • Oral Presentation Immunology Interest Group University of Nebraska Medical Center Omaha, Nebraska "Characterization of RAG complexes supporting synapsis, cleavage, and transposition of V(D)J recombination signals" 2002
  • Poster Presentation 18th UICC Internation Cancer Congress, Oslo, Norway "A molecular characterization of RAG-1/RAG-2 synaptic complexes involved in V(D)J recombination" 2002
  • Third Annual Meeting: Great Plains States Society for Molecular Biology and Genetics;"12/23-Regulated Synapis,Cleavage, and Transposition of V (D)J Recombination Signals is Supported by a RAG-1/2 Tetramer Complex" Abstract and Poster Presentation 2002
  • Keystone Symposium on Molecular Mechanisms of DNA Replication and Recombination, Snowbird,UT"Comparison of catalytic activity and protein-DNA contacts in discrete RAG-RSS complexes lacking or containing HMG-1 or HMG-2" Abstract and Poster presentation 2002
  • AAAS Southwest and Rocky Mountain Division Meeting, Creighton University, "Assembly and Function of V (D)J Initiation Complexes" 2001
  • Nucleic Acids Interest Group, Mahoney State Park "Assembly and function of V(D)J initiation complexes" Oral Presentation 2001
  • American Cancer Society Relay for Life; Norfolk, Nebraska; Introductory remarks in opening ceremony on the importance of cancer research and brief description my ACS funded research 2001
  • Great Plains States Society for Molecular Biology and Genetics "Incorporation of HMG-1 or HMG-2 into V(D)J recombination pre-initiation complexes assembled on 23-RSS substrates stabilizes protein-DNA contacts at the heptamer and correlates with enhanced DNA cleavage activity" Poster Presentation 2001
  • AAAS SWARM Meeting "The V(D)J recombinase cleaves DNA in trans" Oral Presentation 2000
  • Immunology Interest Group Meeting, UNMC "Roles of RAG-1 and RAG-2 in V(D)J recombination" Oral Presentation 2000
  • Oklahoma Medical Research Foundation, Oklahoma City, OK "The roles of RAG-1 and RAG-2 in the recognition and cleavage of V(D)J recombination signals" Invited Presentation 2000
  • Immunology 2000, Seattle, WA "The V(D)J recombinase cleaves DNA in trans" Poster and Oral Presentation 2000
  • Carleton College, Northfield, MN "The assembly of the antigen receptor genes: how RAG-1 and RAG-2 initiate V(D)J recombination" Invited Presentation Poster and Oral Presentation 2000
  • St. Olaf College, Northfield, MN "The assembly of the antigen receptor genes: how RAG-1 and RAG-2 initiate V(D)J recombination" Invited Presentation Poster and Oral Presentation 2000
  • VA Hospital 1999
  • Distinct Roles for RAG-1 and RAG-2 in V(D)J Recombination Signal Recognition and Initiation Complex Assembly Keystone Symposium on B Lymphocyte Biology and Disease February 1999 1999

Awards

  • Intramural Research Award
    Golden Apple Award Nomination for Excellence in Teaching by M1 students
  • Intramural Research Award
    Young Investigator Award Creighton University School of Medicine
  • Intramural Research Award
    Albert Lehninger Research Prize, Young Investigators' Day, The Johns Hopkins University School of Medicine, April 1999