Ronald A . Shikiya, PhD

Department

Med. Microbiology & Immunology

Position

Research Assistant Professor

Books

• Not Applicable
  Prions and Diseases Volume 1, Physiology and Pathophysiology Chapter: Prion Strain Interference Charles R. Schutt, Ronald A. Shikiya and Jason C. Bartz ISBN: 978-1-4614-5304-8 (Print) ISBN: 978-1-4614-5305-5 (Online)
  Springer; 2013 edition, p. 193-204 2012

Articles

• Journal of Biological Chemistry
  1.   Matthew E.C. Bourkas, Hamza Arshad, Zaid A.M. Al-Azzawi, Ondrej Halgas, Ronald A. Shikiya, Mohadeseh Mehrabian, Gerold Schmitt-Ulms, Jason C. Bartz, and Joel C. Watts “Creation of a murine cell line for the propagation and molecular analysis of hamster prions” J. Biol. Chem. 2019. doi:10.1074/jbc.RA118.007135 2019
• PLoS Pathogens
  Thomas E. Eckland; Ronald A. Shikiya; Jason C. Bartz. “Independent amplification of co-infected long incubation period low prion conversion efficiency strains” PLoS Pathogens 2018. https://doi.org/10.1371/journal.ppat.1007323 2018
• Carr CE, Ganugula R, Shikiya R, Soto AM, Marky LA"Effect of dC → d(m5C) substitutions on the folding of intramolecular triplexes with mixed TAT and C+GC base triplets"Biochimie. 2018 Mar;146:156-165. doi: 10.1016/j.biochi.2017.12.008. Epub 2017 Dec 24
  146, p. 156-165 2018
• PLoS Pathogens
  Shikiya RA, Langenfeld KA, Eckland TE, Trinh J, Holec SA, Mathiason CK, Kincaid AE, Bartz JC."PrPSc formation and clearance as determinants of prion tropism"PLoS Pathog. 2017 Mar 29;13(3):e1006298. doi: 10.1371/journal.ppat.1006298. eCollection 2017 Mar.
  13(3) 2017
• Journal of Virology
  J Virol. 2016 May 27;90(12):5715-23. doi: 10.1128/JVI.00409-16. Print 2016 Jun 15.Incongruity between Prion Conversion and Incubation Period following Coinfection.Langenfeld KA1, Shikiya RA1, Kincaid AE2, Bartz JC3.
  90(12) 2016
• Biochimica et Biophysica Acta
  Biochim Biophys Acta. 2016 May;1860(5):990-8. doi: 10.1016/j.bbagen.2015.10.002. Epub 2015 Oct 9.Application of differential scanning calorimetry to measure the differential binding of ions, water and protons in the unfolding of DNA molecules.Olsen CM1, Shikiya R1, Ganugula R1, Reiling-Steffensmeier C1, Khutsishvili I1, Johnson SE1, Marky LA2.
  1860(5) 2016
• PLoS One
  Melissa D. Clouse, Ronald A. Shikiya, Jason C. Bartz, Anthony E. Kincaid “Nasal Associated Lymphoid Tissue of the Syrian Golden Hamster Expresses High Levels of PrPC” PLoS One. 10(2) 2015. doi:10.1371/journal.pone.0117935.
  10(2) 2015
• Prion
  Prion formation, but not clearance, is supported by protein misfolding cyclic amplification. Shikiya RA, Eckland TE, Young AJ, Bartz JC doi: 10.4161/19336896.2014.983759
  8(6) 2014
• Prion
  Ronald A. Shikiya, Thomas E. Eckland, Alan J. Young, and Jason C. Bartz. “Prion formation, but not clearance, is supported by protein misfolding cyclic amplification” Prion 8(6): 1-6. 2014. http://dx.doi.org/10.4161/19336896.2014.983759
  8(6) 2014
• BioScience
  The Story of Prions’ Discovery Shikiya, Ronald A. doi: 10.1093/biosci/biu158
  64(11), p. 1052-1053 2014
• Springer Publishing
  Charles R. Schutt, Ronald A. Shikiya, Jason C. Bartz. “Prions and Diseases: Prion Strain Interference” Springer Science + Business Media. New York. 2013. ISBN: 9781461453048 2013
• Journal of Visualized Experiment
  Protein misfolding cyclic amplification of prions Saunders SE1, Bartz JC, Shikiya RA doi:10.3791/4075.
  69, p. pii: 4075 2012
• Journal of Visualized Experiment
  Samuel E. Saunders, Jason C. Bartz, and Ronald A. Shikiya. “Protein misfolding cyclic amplification of prions” Journal of Visualized Experiments. 2012 Nov 7; 69. pii: 4075. doi: 10.3791/4075.
  69 2012
• Journal of Virology
  In vitro generation of high-titer prions. Shikiya RA, Bartz JC doi: 10.1128/JVI.06134-11
  85(24), p. 13439-42 2011
• Journal of Virology
  Ronald A. Shikiya and Jason C. Bartz. “In Vitro Generation of High-Titer Prions” Journal of Virology. 2011. 85(24). 13439-13442. Doi: 10.1128/JVI.06134-11
  85(24) 2011
• Journal of Virology
  Replication efficiency of soil-bound prions varies with soil type. Saunders SE1, Shikiya RA, Langenfeld K, Bartelt-Hunt SL, Bartz JC doi: 10.1128/JVI.00282-11
  85(11), p. 5476-82 2011
• Journal of Virology
  Replication efficiency of soil-bound prions varies with soil type. Saunders SE1, Shikiya RA, Langenfeld K, Bartelt-Hunt SL, Bartz JC doi: 10.1128/JVI.00282-11
  85(11), p. 5476-82 2011
• Journal of Virology
  Samuel E. Saunders, Ronald A. Shikiya, Katie A. Langenfeld, Shannon L. Bartelt-Hunt, and Jason C. Bartz. “Replication Efficiency of Soil-Bound Prions varies with Soil Type” Journal of Virology. 2011. 85(11). 5476-5482. Doi: 10.1128/JVI.00282-11
  85(11), p. 5476-82 2011
• PLoS Pathogens
  Jacob I. Ayers, Charles R. Schutt, Ronald A. Shikiya, Adriano Aguzzi, Anthony E. Kincaid, Jason C. Bartz. “The Strain-Encoded Relationship between PrPSc Replication, Stability and Processing in Neurons is Predictive of the Incubation Period of Disease” PLoS Pathogens. 2011. 7(3). e1001317.
  7(3) 2011
• PLoS Pathogens
  The strain-encoded relationship between PrP replication, stability and processing in neurons is predictive of the incubation period of disease. Ayers JI, Schutt CR, Shikiya RA, Aguzzi A, Kincaid AE, Bartz JC doi: 10.1371/journal.ppat.1001317
  7(3) 2011
• Journal of Virology
  Coinfecting prion strains compete for a limiting cellular resource. Shikiya RA1, Ayers JI, Schutt CR, Kincaid AE, Bartz JC doi: 10.1128/JVI.00243-10
  84(11), p. 5706-14 2010
• Journal of Virology
  Ronald A. Shikiya, Charles Schutt, Jacob Ayer, Anthony Kincaid, and Jason Bartz. “Co-infecting Prion Strains Compete for a Limiting Cellular Resource” The Journal of Virology. 2010. 84(11) 5706-5714. Doi: 10.1128/JVI.00243-10
  84(11) 2010
• John Wiley & Sons, Inc
  Luis A. Marky, Souvik Maiti, Chris Olsen, Ronald Shikiya, Sarah E. Johnson, Mahima Kaushik, Irine Khutsishvili. “Building Blocks of Nucleic Acid Nanostructures: Unfolding Thermodynamics of Intramolecular DNA Complexes” Biomedical Applications of Nanotechnology. John Wiley & Sons, Inc. 2007. ISBN: 9780471722427 2007
• Biochemistry
  Ana Maria Soto; Dionisios Rentzeperis; Ronald Shikiya; Manuel Alonso; Luis A. Marky. “DNA Intramolecular Triplexes Containing dT→dU Substitutions: Unfolding Energetics and Ligand Binding” Biochemistry. 2006. 45(9) 3051-3059
  45(9) 2006
• Journal of Physical Chemistry B
  Ronald Shikiya and Luis A. Marky, “Calorimetric unfolding of intramolecular triplexes: length dependence and incorporation of single AT -> TA substitutions in the duplex domain.” J Phys Chem B. 2005. 109(38) 18177-18183
  109(38) 2005
• Biochemistry
  Ronald Shikiya, Jian-Sen Li, Barry Gold, and Luis A. Marky, “Incorporation of cationic chains in the Dickerson-Drew dodecamer: correlation of energetics, structure, and ion and water binding”. Biochemistry. 2005. 44(37) 12582-12588
  44(37), p. 12582-12588 2005
• Journal of American Chemical Society
  Jian-Sen Li, F. Cheng, Ronald Shikiya, Luis A. Marky, and Barry Gold. “Molecular recognition via triplex formation of mixed purine/pyrimidine DNA sequences using oligoTRIPs”. J. Am. Chem. Soc. 2005. 127(36) 12657-12665
  127(36) , p. 12657-12665 2005
• ACS Symposium Book Series
  Luis A. Marky, Karen Alessi, Ronald Shikiya, Jian-Sen Li, and Barry Gold. “Folding of Bent Oligodeoxynucleotide Duplexes: Energetics, Ion Binding and Hydration”. Nucleic Acids: Curvature and Deformation. ACS Symposium Series Book Chapter. Oxford University Press. September 2004. ISBN: 9780841238626 2004
• Biochemistry
  Jian-Sen Li, Ronald Shikiya, Luis A. Marky, and Barry Gold. “Triple Helix Forming TRIPside Molecules That Target Mixed Purine/Pyrimidine DNA Sequences”. Biochemistry, 2004. 43(6) 1440-1448
  43(6) , p. 1440-1448 2004
• Biopolymers
  Besik I. Kankia, Ana Maria Soto, Nicole Burns, Ronald Shikiya, Chang-Shung Tung, and Luis A. Marky. “DNA Oligonucleotide Duplexes Containing Intramolecular Platinated Cross-Links: Energetics, Hydration, Sequence, and Ionic Effects”. Biopolymers, 2002. 65(3). 218-227
  65(3), p. 218-227 2002
• Journal of Physical Chemistry B
  Dionisios Rentzeperis, Ronald Shikiya, Souvik Maiti, James Ho, and Luis A. Marky. “Folding of Intramolecular DNA Hairpin Loops: Enthalpy-Entropy Compensations and Hydration Contributions”. Journal of Physical Chemistry B, 2002. 106(38) 9945-9950
  106(38), p. 9945-9950 2002

Publications

• Prion
  Restricted propagation of sheep scrapie in hamsterhttp://dx.doi.org/10.1080/19336896.2016.1162644 
  10(Sup1) 2016

Presentations

• "PrPSc formation and clearance as determinants of prion tropism" Prion 2018 Santiago de Compostela, Spain 2018
• NES 510 Neurophysiology Lab, Guest Speaker. Creighton University. Omaha, NE 2018
• "Persistence of high titer prion strain in a prion strain mixture". INBRE 2017. August 6-8, 2017. 2017
• "Incongruity between prion conversion and incubation period following co-infection". Prion 2017. May 23-26, 2017. Edinburgh, Scotland. 2017
• NES 510 Neurophysiology Lab, Guest Speaker. Creighton University. Omaha, NE 2017
• PHY 353 Introduction to Biological Physics, Guest Speaker. Creighton University. Omaha, NE 2017
• "Restricted propagation of sheep scrapie in hamsters" Prion 2016. May 10-13, 2016. Tokyo, Japan. 2016
• "Prion formation, but not clearance, is supported by PMCA" Prion 2015. May 26-29. Fort Collins, CO 2015
• "Abundance of prion proteins in Lymphoreticular tissue". 2014 Nebraska Neuroscience Symposium. September 24th, 2014. Omaha, NE 2014
• "Absence of PrPSc clearance in PMCA". Expanding Prion Horizons 2013. October 16th-18th, 2013. Fort Collins, CO. 2013
• "Investigation of conversion efficiency between natural and in vitro generated prions". Expanding Prion Horizons 2013. October 16th-18th, 2013. Fort Collins, CO. 2013
• "Endogenous PrPC Levels in Hamster Lymphoid Tissue". Expanding Prion Horizons 2013. October 16th-18th, 2013. Fort Collins, CO 2013
• "Prion Strain Emergence". Neuro-Prion 2013 Annual Conference. May 26th-29th, 2013. Banff, Canada 2013
• "In Vitro Generation of High-Titer Prions". Neuro-Prion 2012 Annual Conference. May 9th-12th, 2012. Amsterdam, The Netherlands. 2012
• "The Strain-encoded Relationship Between PrPSc Replication, Stability and Processing in Neurons is Predictive of the Incubation Period of Disease". Neuro-Prion 2011 Annual Conference. May 17th-19th, 2011. Montreal, Canada 2011
• PHY 353 Introduction to Biological Physics, Guest Speaker. Creighton University. Omaha, NE 2011
• "Amplification Coefficient of Hamster Prion Strains". Neuro-Prion 2010 Annual Conference. September 8th-11th, 2010. Salzburg, Austria 2010
• PHY 353 Introduction to Biological Physics, Guest Speaker. Creighton University. Omaha, NE 2010
• "In vitro Prion Strain Interference". Neuro-Prion 2008 Annual Conference. October 8th-10th, 2008. Madrid, Spain. 2008
• "In vitro Amplification of Prion Strains". Neuro-Prion 2007 Annual Conference. September 24th-28th, 2007. Edinburgh, Scotland 2007

Foundation/Association

• Protein conformational dynamics as indicator of interspecies transmission in prion diseases