Michael A . Belshan, PhD
Professor
Medical Microbiology and Immunology
Contact
School of Medicine
Med Microbiology & Immunology
Medical Microbiology and Immunology (Doctorate)
Medical Microbiology and Immunology (Master of Science)
Graduate School
CRISS II - Criss 2 - 514C
Michael A . Belshan, PhD
Professor
Medical Microbiology and Immunology
Examples of the most recent publications can be found at this PubMed link.
Research Focus
HIV Molecular Virology and Host Cell InteractionsMy fundamental research interest is virus-host cell interactions. The majority of my work is related to the replication and pathogenesis of the lentivirus subfamily of retroviruses, including Human Immunodeficiency Virus (HIV) and Simian Immunodeficiency Virus (SIV). However, I have a broad background in virology, molecular biology and OMICs studies. I have worked in the field of virology for over 25 years and have an established record of productive research in the areas of molecular virology, virus-host interactions, viral evolution, proteomic studies, and in vitro drug testing.
HIV is the causative agent of acquired immunodeficiency syndrome (AIDS). HIV infection is incurable, but treatment with a combination of anti-retroviral drugs can reduce virus replication to undetectable levels and forestall disease progression. However drug resistance to the existing pool of anti-retroviral therapies remains a concern. Continued success in the repression of HIV replication in infected individuals will require the development of new inhibitors that are effective against drug-resistant strains of virus. Drugs that target novel areas of virus replication have the greatest probability to be effective against such viruses.
My laboratory seeks to comprehend poorly understood aspects of HIV replication by discovery of novel virus-host interactions using systems biology approaches. The ultimate goal is to identify new targets for anti-viral therapies. All areas of HIV replication can have potential cellular targets, including virus uncoating, preintegration complex (PIC) assembly and transport, viral transcriptional regulation, and virus assembly and release.
Major Areas of Research
- OMICs studies to identify novel virus-host interactions.
- Characterization of novel host cell factors that contribute to HIV replication, latency, and pathogenesis.
- Molecular biology of HIV replication, latency, and pathogenesis
- In vitro drug testing.
Department
Med. Microbiology & Immunology
Position
Professor
Books
- Reference Module in Biomedical Research
Belshan Michael A., Poliomyelitis [Book Chapter] 2014 - Reference Module in Biomedical Research
Belshan Michael A., Influenza A [Book Chapter] 2014 - Reference Module in Biomedical Research
Belshan Michael A., Rabies [Book Chapter] 2014
Articles
- Kovarova Martina, Correction to 2015
Publications
- HGG advances
Plowman Jocelyn N, Targeted sequencing for hereditary breast and ovarian cancer in BRCA1/2-negative families from the Lynch Memorial Biobank reveals complex genetic architecture and phenocopies, p. 100306 - 100306 2024 - HGG advances
Matoy Evanjalina J., In vitro data suggest a role for PMS2 Kozak sequence mutations in Lynch Syndrome risk, p. 100298 - 100298 2024 - Nature communications
Deng Silu, Cryptosporidium uses CSpV1 to activate host type I interferon and attenuate antiparasitic defenses
14:1, p. 1456 - 17 2023 - Nanomaterials
Mandal Subhra, Targeted Immuno-Antiretroviral to Promote Dual Protection against HIV
12:11 2022 - Frontiers in Microbiology
Siedlik Jacob A., Epidemiologic and Genomic Analysis of the Severe Acute Respiratory Syndrome Coronavirus 2 Epidemic in the Nebraska Region of the United States, March 2020–2021
13 2022 - Proteomics
Macur Katarzyna, SWATH-MS and MRM
21:15 2021 - Virology
Belshan Michael, Discovery of candidate HIV-1 latency biomarkers using an OMICs approach
558, p. 86 - 95 2021 - PloS one
Tso For Yue, Presence of antibody-dependent cellular cytotoxicity (ADCC) against SARS-CoV-2 in COVID-19 plasma
16:3 March 2021 - Physiological Reports
Holbrook Alexander K., CD4+ T cell activation and associated susceptibility to HIV-1 infection in vitro increased following acute resistance exercise in human subjects
7:18 2019 - Antiviral Research
Mandal Subhra, A potential long-acting bictegravir loaded nano-drug delivery system for HIV-1 infection
167, p. 83 - 88 2019 - Medicine and science in sports and exercise
Bianchi Samantha A., Cd28 Expression On Cd4+ T Cells Is Not Affected By Strenuous Exercise In Untrained Individuals
50:5S, p. 473 - 473 2018 - Medicine and science in sports and exercise
Holbrook Alexander K., The Effect Of Exercise On CD4+ T-cell Activation And Their Susceptibility To HIV-1
50:5S, p. 224 - 224 2018 - Virology
DeBoer Jason, Proteomic profiling of HIV-infected T-cells by SWATH mass spectrometry
516, p. 246 - 257 2018 - Antimicrobial Agents and Chemotherapy
Mandal Subhra, An Enhanced Emtricitabine-Loaded Long-Acting Nanoformulation for Prevention Or Treatment of HIV Infection
61, p. e01475 - 16 2017 - Virology
DeBoer Jason, Cyclophilin B enhances HIV-1 infection
489, p. 282 - 291 2016 - Proteomics.Clinical Applications
Li Yan, Investigation of the HIV-1 matrix interactome during virus replication
10:2, p. 156 - 163 2016 - Viruses
Li Yan, NF45 and NF90 Bind HIV-1 RNA and Modulate HIV Gene Expression
8:2, p. (17 pages) 2016 - PLoS Pathogens
Kovarova M., Nanoformulations of Rilpivirine for Topical Pericoital and Systemic Coitus-Independent Administration Efficiently Prevent HIV Transmission
11:8, p. (19 pages) 2015 - Journal of Biomedical Nanotechnology
Date Abhijit A., Thermosensitive Gel Containing Cellulose Acetate Phthalate-Efavirenz Combination Nanoparticles for Prevention of HIV-1 Infection
11:3, p. 416 - 427 2015 - Virology
DeBoer Jason, Alterations in the nuclear proteome of HIV-1 infected T-cells
468-470, p. 409 - 420 2014 - Virology
Sanford Bridget Lanelle, Deletions in the fifth alpha helix of HIV-1 matrix block virus release
468–470, p. 293 - 302 2014 - AIDS Research and Human Retroviruses
Shibata Annemarie, Erratum: Polymeric Nanoparticles Containing Combination Antiretroviral Drugs for HIV Type 1 Treatment (AIDS Research and Human Retroviruses (2013) 29:5 DOI: 10.1089/aid.2012.0301))
29:7, p. 1095 2013 - Retrovirology
Gérard Annabelle, Identification of low molecular weight nuclear complexes containing integrase during the early stages of HIV-1 infection
10:1 2013 - Journal of Proteome Research
Schweitzer Cameron J., Proteomic Analysis of Early HIV-1 Nucleoprotein Complexes
12:2, p. 559 - 572 2013 - AIDS Research and Human Retroviruses
Shibata Annemarie, Polymeric nanoparticles containing combination antiretroviral drugs for HIV type 1 treatment
29:5, p. 746 - 754 2013 - Journal of Virology & Antiviral Research
Belshan Michael, HIV Research Beyond the “omics” Era
1:1 2012 - PLoS One
Schweitzer Cameron J., Knockdown of the Cellular Protein LRPPRC Attenuates HIV-1 Infection
7:7, p. e40537 2012 - Retrovirology
Belshan Michael A., Vpx is Critical for SIVmne infection of pigtail macaques
9, p. 32 - 32 2012 - Antiviral Research
Date Abhijit A., Development and evaluation of a thermosensitive vaginal gel containing raltegravir + efavirenz loaded nanoparticles for HIV prophylaxis
96:3, p. 430 - 436 2012 - Journal of Virological Methods
Belshan Michael A., Replication of biotinylated human immunodeficiency viruses
171:1, p. 299 - 302 2011 - Journal of Antimicrobial Chemotherapy
Destache Christopher J., Antiretroviral release from poly(DL-lactide-co-glycolide) nanoparticles in mice
65:10, p. 2183 - 2187 2010 - Journal of Virological Methods
Belshan Michael A., In vivo biotinylation and capture of HIV-1 matrix and integrase proteins
159:2, p. 178 - 184 2009 - Journal of Virology
Cheng Xiaogang, Hsp40 facilitates nuclear import of the human immunodeficiency virus type 2 Vpx-mediated preintegration complex
82:3, p. 1229 - 1237 2008 - Virology
Mahnke Lisa A., Analysis of HIV-2 Vpx by modeling and insertional mutagenesis
348:1, p. 165 - 174 2006 - Journal of Virology
Lee Jae Hyung, Characterization of functional domains of equine infectious anemia virus Rev suggests a bipartite RNA-binding domain
80:8, p. 3844 - 3852 2006 - Virology
Belshan Michael, Conserved amino acids of the human immunodeficiency virus type 2 Vpx nuclear localization signal are critical for nuclear targeting of the viral preintegration complex in non-dividing cells
346:1, p. 118 - 126 2006 - Journal of Virology
Baccam Prasith, Subpopulations of Equine Infectious Anemia Virus Rev Coexist In Vivo and Differ in Phenotype
77:22, p. 12122 - 12131 2003 - Virology
Belshan Michael, Identification of the nuclear localization signal of human immunodeficiency virus type 2 Vpx
311:1, p. 7 - 15 2003 - Virology
Belshan Michael, Genetic and biological variation in equine infectious anemia virus Rev correlates with variable stages of clinical disease in an experimentally infected pony
279:1, p. 185 - 200 2001 - Molecular and Cellular Biology
Belshan Michael, Binding of equine infectious anemia virus Rev to an exon splicing enhancer mediates alternative splicing and nuclear export of viral mRNAs
20:10, p. 3550 - 3557 2000 - Journal of Virology
Belshan Michael, Biological characterization of Rev variation in equine infectious anemia virus
72:5, p. 4421 - 4426 1998
Presentations
- HIV: Quest for a cure 2018
- CypB stimulates HIV infection 2015
- TM261: A Viral Study Led Astray 2015
- Proteomic Approaches to Study HIV-Host Interactions 2014
- SWATH-MS Analysis of HIV-1 Infected Jurkat T-cells 2014
- Inbre Student Jessica Haight in Grand Island, NE 2006
Awards
- Young Investigator Award
Creighton University School of Medicine - Research Excellence Award
Iowa State University Graduate College
Consulting
- Infectious Disease Scientific Director
Advisory
Nebraska Institutional Development Awards (IDeA) Networks of Biomedical Research Excellence (INBRE Program)
2021 - 2025